Figure 2. Genes for lipid synthetic pathways and zinc-binding proteins are prominent among DEGs in ARPE-19 in serum-free medium. A: Gene expression results measured by microarrays for serum deprived ARPE-19 cells compared to Day0 serum supplemented cultures. Bars represent the ratio of gene expression. Results are filtered for p values less than 0.05 and fold-change values greater than 1.2. The top 10 up and downregulated genes are shown. The bars are colored to identify genes classified as cholesterol/lipid or Zn-binding. Interactive figures, using Tableau Software (Seattle, WA), for the complete DEG (Interactive Figures). B: Comparison of the effect of serum deprivation on gene expression in ARPE-19 versus human embryonic kidney (HEK293T) cells. Key genes in the cholesterol/lipid pathway have increased expression at 3 days in serum-free medium (SFM) in ARPE-19 cells but not in HEK293T cells. Gene expression is measured as the ratio of day 0 serum-supplemented cultures and serum-starved cultures with microarray hybridization.