Figure 1. RPE-derived cells survive well in serum-free medium. A: The cell survival percentage of ARPE-19 cells (solid line) and human embryonic kidney (HEK293) cells (dashed line) under
serum deprivation with standard deviation error bars, compared with day 0 (100%). B: Expression of mRNAs of RPE markers, RPE65 and RLBP1, were detected in ARPE-19 cells in serum-free medium (SFM) with PCR.
NT is no template control. C: HEK293 cells die by apoptosis in SFM, ARPE-19 and HEK293 cells were serum starved, and terminal deoxynucleotidyl transferase
dUTP nick end labelin (TUNEL) assay was performed. Confocal Differential interference contrast (DIC; left) and green fluorescence
(right) TUNEL images show unstained and apoptotic cells, respectively. Scale bar=20 μM.