Figure 2. Comparison of growth and differentiation markers in primary- and tertiary-generation explant outgrowths. A and B: p63 immunostaining. C and D: Krt3 immunostaining. A and C: Primary cultures. B and D: Tertiary-generation cultures. E: Western blots. Targets and culture generations are indicated. Bar equals 50 µm in A and B and 33 µm in C and D.