Figure 1. Scatter and JC1 exclusion in cells grown on hAM or Falcon substrata. A–C: Cells from explants cultured over human amniotic membrane (hAM). D–F: Cells from cultures on synthetic membrane. A and D: Scatter plots. B–F: JC1 bivariate emission dot plots. Note the distinct shape of the main cell cohort. C and F: Contour plots. The demarcated area at the center of the plot was used to determine the emission intensities and the intensity ratios described in Table 1.