Figure 5. Histological analysis of inhibition of ischemia-induced retinal neovascularization by siRNA targeting Islet-1. A: Room air–raised mice. B: The murine model of oxygen-induced retinopathy (OIR). C: The murine OIR model with injection of negative control siRNA. D: The murine OIR model injected with siRNA targeting insulin gene enhancer protein ISL-1 (Islet-1). E: Retinal neovascularization was assessed histologically by counting the endothelial cell nuclei protruding into the vitreous cavity. The mean number of neovascular cell nuclei in eyes injected with Islet-1 siRNA was approximately 50% of the number in the eyes of the murine model of OIR (★p<0.05). GCL = ganglion cell layer; INL = inner nuclear layer; ONL= outer nuclear layer.