Figure 2. Effect of PKR siRNA and PI3K inhibitor LY294002 on hypoxia-induced PKR, p-PI3K, p-Akt, and VEGF expression in RF/6A cells.
A: DsRNA-activated protein kinase (PKR), phosphophosphatidylinositol 3-kinase (p-PI3K), phosphoprotein kinase B (p-Akt), and
vascular endothelial growth factor (VEGF) expression was detected with western blotting in the RF/6A cells following PKR siRNA
transfection after 48 h. C: The histogram shows the densitometric analysis of the average levels for PKR, p-PI3K, p-Akt, and VEGF to GAPDH. Cells transfected
with scramble siRNA were used as the negative control. B: The western blot shows PKR, p-PI3K, p-Akt, and VEGF expression in RF/6A cells treated with the PI3K inhibitor LY294002 for
30 min. D: The histogram shows the densitometric analysis of the average levels of PKR, p-PI3K, p-Akt, and VEGF to GAPDH. Hypoxic cells
were used as the negative control. *p<0.05, statistically significantly different compared to the respective controls. Values
represent means ± SD.