Table 1 of Daruich, Mol Vis 2016; 22:1332-1341.
Protocol | Model | Main Results | Ref | ||
---|---|---|---|---|---|
Injected compound | Dose | Time of sacrifice | |||
NMDA | 30 nM | 12 h after injection | tPA −/− and wild-type mice | TUNEL-positive cells in the GCL and INL in tPA−/−mice after intravitreal injection of NMDA were significantly fewer than in wild-type mice | [37] |
NMDA | 30, 120 and 240 nM | 6, 12, 24, 72 h, 1 or 2 weeks | tPA −/− and wild-type mice | At 12 h, TUNEL-positive cells in both GCL and INL were significantly fewer in tPA-deficient mice than in wild-type mice, only in the case of the lower dose of NMDA (30 nmol/mouse), but not at higher doses (120 or 240 nmol/mouse) | [17] |
Kainic acid (KA) | 10, 20, and 40 nM | 48 h | Adult CD-1 mice | KA-injected eyes showed a dose-dependent and time-related increase in tPA activity in the retina, witch was associated with TUNEL-positive cells the GCL and subsequently in the INL and ONL. | [18] |
Commercial rtPA containing L-Arginine (Actilyse ®) | 1 μl of 250 μg/ml tPA (containing L-arginine) | 24 h | ICR mice | Severe morphologic damages appeared in the retinal cell layers with a vitreous injection of the tPA (containing L-arginine) compared to the control eye | [46] |
5 mM of L-arginine alone | Similar histopathologic results were observed in the retinal areas receiving L-arginine (5 mM) | ||||
10 μg/400 μl tPA (containing L-arginine) | - | Primary retinal cells culture | Exposure of cultured retinal cells to tPA (containing L-arginine) or L-arginine led to a marked increase in nitrite, a nitric oxide intermediator |