Figure 2. Flatmounted retinas from rats euthanized 24 h after intravitreal administration of rtPA (2.16 µg/5 µl) or vehicle (NaCl 0.9%, 5 µl). Apoptotic retinal ganglion cells labeled with intravitreal annexin V-fluorescein isothiocyanate (FITC) (A–C) or annexin V-FITC and 4',6-diamidino-2-phenylindole (DAPI) (B–D) before the animals were euthanized. A, B: Control eye (NaCl 0.9%, 5 µl). C, D: Eye treated with intravitreal recombinant tissue plasminogen activator (rtPA; 2.16 µg/5 µl) displaying a higher number of annexin V-FITC labeling, colocalizing with retinal ganglion cell nuclei. Green = Annexin-V-FITC staining; Blue = DAPI staining. Bar = 50 µm.