Molecular Vision: Markand, Mol Vis 2016; 22:1291-1308. Table 2

Table 2. Excessive axial length in the KO originates solely from an increased vitreous chamber depth.

Dimension	Mean	SD	Sample number	Mean	SD	Sample number	ANOVA p-value Tukey	Significant at p<0.05?
	Wild type P30			Knockout P30
CCT	100.1	10.58	8	109.4	9.18	6	ns	No
ACD	289.6	12.49	8	259.4	14.14	6	ns	No
Lens	1800.9	41.2	8	1787	29.19	6	ns	No
Vitreous	638.4	37.59	8	1018.4	51.26	6	< 0.0001	Yes
Retina	210.5	24.94	8	172	16.39	6	< 0.05	Yes
Total axial length	3067.6	56.52	8	3353.9	35.44	6	< 0.0001	Yes
	Wild type P45			Knockout P45
CCT	87.9	12.13	7	90.27	9.07	8	ns	No
ACD	318.1	15.13	7	296.04	15.99	8	ns	No
Lens	1918.6	38	7	1920.62	30.86	8	ns	No
Vitreous	653.7	15.77	7	971.27	52.03	8	< 0.0001	Yes
Retina	208.9	15.77	7	183.37	15.48	8	ns	No
Total axial length	3224	55.22	7	3475.17	27.58	8	< 0.0001	Yes
	Wild type P53-55			Knockout P53-55
CCT	111	4.4	3	112.6	5.397	5	ns	No
ACD	314.5	4.49	3	294.7	10.92	5	ns	No
Lens	1866	14.93	3	1849	16.74	5	ns	No
Vitreous	564	8.73	3	899.6	15.12	5	< 0.0001	Yes
Retina	274.1	12.7	3	239.4	27.7	5	ns	No
Total axial length	3130	9.91	3	3396	36.34	5	< 0.0001	Yes
	Wild type P80-82			Knockout P80-82
CCT	87.3	7.44	5	95.5	7.61	7	ns	No
ACD	365.2	26.35	5	354.7	33.19	7	ns	No
Lens	2099	81.37	5	2075.5	32.13	7	ns	No
Vitreous	530.3	60.93	5	883.8	18.17	7	< 0.0001	Yes
Retina	222	13.84	5	175	19.59	7	< 0.05	Yes
Total axial length	3359.9	34.21	5	3615	63.63	7	< 0.0001	Yes

Axial measurements were made with a Bioptigen 4310. Axial measurements were collected in the KO versus WT mouse eyes at P30, P45, P54, and P80. All measurements are in microns. ANOVA and a Tukey test were conducted. The essential point of these axial measurements is that the vitreous chamber of the KO was much deeper than the WT, and this accounted for all the excess axial length of the KO mouse. Abbreviations: CCT, central corneal thickness; ACD, anterior chamber depth; Lens, lens thickness; Vitreous, Vitreous Chamber depth; Retina, retina thickness; Length, total axial length from anterior surface of the cornea to the basal face of the RPE.