Figure 4. Compstatin peptides inhibit complement-induced C5b-9_n deposition in RPE culture. Fluorescence intensity measurements from anti-C5b-9_n-labeled (decellularized) whole mount samples (n ≥ 9) were normalized human serum (NHS)-treated samples and plotted as mean ± standard error of the mean (SEM). Data analyses show that Peptide 2 causes 80% reduction in NHS-induced basal C5b-9_n deposition by RPE cells. Peptide 1 exhibits a weaker inhibitory effect, reducing C5b-9_n deposition by 50%. Bars indicate mean ± SEM.