Figure 1. Analysis of RNA integrity in human LG and SMG tissues. Total RNA was extracted from lacrimal gland (LG) and submandibular gland (SMG) tissue from three donors (D1, D2, and D3) and used to investigate RNA integrity. A: Representative Bioanalyzer 2100 traces from human LG and SMG total RNA depicting high RNA integrity number (RIN) values. B: Extracted RNA was used for reverse transcriptase PCR analysis of E-cadherin, aquaporin 5, and GAPDH (the control housekeeping gene).