Figure 4. The effect of TTR on tube formation process of hRECs. Human retinal microvascular endothelial cells (hRECs) were cultured
in natural and simulated diabetic retinopathy (DR) media with TTR, and in vitro tube formation assay was performed. A: The photos were scanned by the NIH Image program, and the total tube length was calculated. B: In the low-glucose media (LG and LG+Hypoxia), the tube length was enhanced by TTR (*: p = 0.009, F = 22.311; #: p < 0.001,
F = 465.592); while in the high-glucose media (HG and HG+Hypoxia), the tube length was decreased with TTR (**:p < 0.001, F
= 933.495; ##: p < 0.001, F = 364.605). (n=3, mean±SD was calculated).