Figure 2. Multiplex real-time analysis of LHON subject samples. A: Unmutated DNA; only the control product is amplified. B: Subject DNA with mutation in 3460A; products at 90.04 °C and 82.32 °C. C: Subject DNA with mutation in 11778A; products at 90.04 °C and 80.12 °C. D: Subject DNA with mutation in 14484C mutation; products at 90.04 °C and 76.32 °C.