Figure 2. TNF-α induces ROS generation within multiple cell types within CNV lesions. A: Immunostaining for oxidized phospholipids (OxPLs) in retinal/choroidal cryosections. Images were taken in the regions with
choroidal neovascularization (CNV) at 20X and 40X magnification. Top panel: 20X, bottom panel: 40X. Green, OxPL; Red, lectin;
Gray, Topro 3. B: Quantification of densitometry of OxPL immunofluorescence at CNV lesions of mice injected with isotype immunoglobulin G
(IgG, 50 ng) or neutralizing tumor necrosis factor alpha (TNF-α) antibody (TNF-α Ab, 50 ng; *p<0.05 versus IgG).