Figure 1. TNF-α mediates CNV formation in association with VEGF expression in a murine model of laser-induced CNV. A: Representative images of western blot of tumor necrosis factor alpha (TNF-α) and vascular endothelial growth factor (VEGF)
protein in RPE/choroids of C57Bl/6 6-week-old mice without laser treatment (non-lasered) or 7 days after laser treatment.
B: Quantification of densitometry (lasered; *p<0.05, **p<0.01 versus non-lasered). C: Representative images of choroidal neovascularization (CNV) volume. D: Quantification of CNV volume. E: Representative images of western blot of VEGF in RPE/choroids of mice injected with isotype immunoglobulin G (IgG, 50 ng)
or neutralizing TNF-α antibody. F: Quantification of densitometry (TNF-α Ab, 50 ng; ††p<0.01 versus IgG).