Figure 7. Proinflammatory cytokines decreased the expression of RPE characteristic genes in an RPE cell culture system established from adult human donor eyes. The cultured cells were treated with the cytokines (interferon gamma (IFN-γ), 100 u/ml + tumor necrosis factor alpha (TNF-α), 10 ng/ml + interleukin-1 beta (IL-1β), 10 ng/ml) for 20 h under serum-free conditions. Real-time PCR analysis of the expression of mRNAs for RDH5, RDH10, MITF, and RPE65 is shown. *p<0.05 when compared to respective control, n = 4.