Figure 5. Proinflammatory cytokines decreased the expression of genes that control RPE function. Differentiated ARPE-19 cells were treated
with the cytokines (interferon gamma (IFN-γ), 100 u/ml + tumor necrosis factor alpha (TNF-α), 10 ng/ml + interleukin-1 beta
(IL-1β), 10 ng/ml) either for 20 h under serum-free conditions or for 4 days in the presence of 1% serum. Real-time PCR was
employed to analyze the expression of the indicated transcripts and miRNAs. *p<0.05 when compared to respective control, n
= 3.