Figure 4. Proinflammatory cytokines decreased the expression of the RLBP1 protein. Differentiated ARPE-19 cells were treated with the cytokines (1X = interferon gamma (IFN-γ), 10 u/ml + tumor necrosis factor alpha (TNF-α), 1 ng/ml + interleukin-1 beta (IL-1β), 1 ng/ml) for 4 days in the presence of 1% serum. A: Western immunoblot analysis of RLBP1 expression. Lane 1: molecular weight markers; lane 2: control; lane 3: cytokines, 1X; and lane 4: cytokines, 10X. A representative blot from three similar experiments is shown. B: Histogram showing RLBP1 expression. The RLBP1 band intensity was normalized with corresponding α-tubulin band intensity; *p<0.05 when compared to control, n = 3.