Figure 9. WFA and Cl-amidine have no effect on insoluble GFAP in the retina. Eyes were injured and left to recover for 7 days in vivo. On day 7, mouse eyes were enucleated and posterior eye-cups were placed into culture with no drug added (lane 1), or containing either Cl-amidine alone (lane 2), WFA alone (lane 3), or both drugs combined (lane 4). A: Western blot analysis of GFAP in insoluble fraction of untreated, Cl-amidine treated, WFA treated, or WFA/Cl-amidine treated eye-cups. Densitometric quantification of high molecular weight (B, >75 kDa), low molecular weight (C, 37-50 kDa), and 50 kDa (D, arrowhead) GFAP species. Data are mean ± standard deviation (SD). P values less than 0.05 were not obtained as determined by t test.