Figure 7. High magnification images of citrullinated vimentin filaments in retinas after ocular injury. Eyes injured for either 3 h or 3 days were sectioned and immunostained for vimentin (A, E, red), anti-citrullinated proteins (B, F, F95 antibody, green) and DAPI (C, G, merged images, blue). A: Vimentin filaments showed staining in the GCL and IPL 3 h postinjury. B: F95 positive filaments showed staining at 3 h post-injury in the GCL and IPL. Filaments in the retina displayed co-localization of F95 and vimentin (C, asterisk; D, asterisk/arrowhead), as well as a distinct interspersed pattern with F95 reactive regions capping filaments near the boundary of the IPL and INL (C, arrow). E: At 3 days postinjury, vimentin-positive filaments showed staining in the IPL and both filamentous and nonfilamentous vimentin immunoreactivity was present in the GCL. F: Diffuse staining and filamentous staining of F95 reactive proteins was present in the GCL and IPL 3 days postinjury. G, H: Vimentin and F95 displayed distinct co-localization (G, asterisk; H, asterisk, arrowhead), as well as an interspersed pattern (G, bracket). D, H: Orthogonal views of xyz stacks were obtained to identify points of colocalization (asterisk, arrowhead). (Scale bar = 40 μm).