Figure 10. WFA and Cl-amidine alter the distribution of vimentin in the retina. Eyes were injured and left to recover for 7 days in vivo. On day 7, mouse eyes were enucleated and posterior eyecups (neural retina, pigment epithelium, choroid) were placed into culture containing specified concentrations of either Cl-amidine (lane 2), WFA (lane 3), both Cl-amidine and WFA (lane 4) or no drug added (lane 1). A: Western blot analysis of vimentin in soluble fractions of untreated, Cl-amidine treated, WFA treated, or WFA/Cl-amidine-treated eyecups. Densitometric quantification of low molecular weight vimentin (B, 37-55 kDa), or 55-kDa vimentin (C, arrowhead). D: Western blot analysis of vimentin in insoluble fraction of untreated, Cl-amidine treated, WFA-treated, or WFA/Cl-amidine-treated eyecups. Densitometric quantification of low molecular weight (B, E, 37-55 kDa) and 55-kDa (C, F, arrowhead) vimentin species. Data are mean ± standard deviation (SD). P values less than 0.05 (asterisk) were considered significant as determined by t test.