Appendix 5 of Wizeman, Mol Vis 2016; 22:1137-1155.


Appendix 5: Co-localization of Citrullination on GFAP Filaments at 3 days

To access the data, click or select the words “Appendix 5.” Plane-by-Plane Confocal Imaging of 3 Day Injured eyes Immunostained for F95 (green), GFAP (red), DAPI (blue). Confocal z-stacks were obtained on a Zeiss 780 Confocal microscope at 63X. Individual planes were then increased in brightness to the same level in Lightroom. Images were re-assembled as a .mov at 0.5 s per focal plane. Images encompass planes containing GFAP staining to highlight co-localization with citrullinated species over the length of glial processes.