Appendix 2 of Wizeman, Mol Vis 2016; 22:1137-1155.


Appendix 2. Overlaid images of GFAP and citrullinated GFAP.

To access the data, click or select the words “Appendix 2.” Mouse eyes were injured and left to recover for 7 days in vivo. On day 7, retinochoroidal tissues from the posterior eyecups were extracted into soluble fractions. The soluble fractions were then either (A) directly separated by 2-dimensional gel electrophoresis or (B) immunoprecipitated for F95 reactive species and then separated by 2-dimensional gel electrophoresis. The protein blots from these analysis were then probed for (A) total GFAP or (B) citrullinated GFAP (CGTF-1221 antibody). The digital images were then overlaid (C) to compare different GFAP species (red) with those that contained citrullinated species (green).