Figure 5. Evaluation of NT4/5 expression in the retina with immunohistochemistry. A: Representative micrographs of retinal sections obtained from each group stained with anti-neurotrophin-4/5 (NT4/5) antibody (red, in the GCL, amplified images in yellow boxes). D: Merged images (in the GCL, amplified images in yellow boxes) of NT4/5 expression (A). B: RNA-binding protein with multiple splicing (Rbpms; green, in the GCL, amplified images in yellow boxes). C: 4’-6-diamidino-2-phenylindole (DAPI; blue, in the GCL, amplified images in yellow boxes). Control = sham-operated animal; ONC = optic nerve crush animal; ALA-ONC = alpha lipoic acid (ALA) animal pretreated 1 day before ONC; ONC-ALA = ALA-treated animal 1 day after ONC. Scale bar = 50 µm. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer.