Figure 7. Differentiation of rod photoreceptors and Müller glia in developing retinal organoids (day (D)18–35). Reep6 (green) and glutamine synthetase (GS, red) are markers of rod photoreceptors and Müller glia, respectively. No significant morphological differences were observed among the neural retinas differentiated from embryonic pluripotent stem cells (ESCs) or induced pluripotent stem cells (iPSCs); representative figures of the neural retinas are shown. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue). Arrowheads indicate the relevant immunostaining with Reep6 and GS. AP and BA show the apical and basal sides of the organoid, respectively. NBL, neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 50 μm.