Figure 6. Levels of p-AKT in the retinas of rats with elevated IOP overexpressing Brn3b. A: Immunostaining for p-AKT in retinal ganglion cells (RGCs) of Brown Norway rats intravitreally injected with either the recombinant adenoassociated virus–cytomegalovirus–green fluorescent protein (rAAV-CMV-GFP) or rAAV-CMV-Brn3b following intraocular pressure (IOP) elevation. Retinal sections obtained were immunostained for p-AKT (pseudogreen) and βIII-tubulin (pseudored). B: An increase in immunostaining (not statistically significant) for p-AKT was observed in RGCs overexpressing Brn3b (rAAV-CMV-Brn3b) compared to RGCs overexpressing the control vector (rAAV-CMV-GFP; determined with the L/R ratios of fluorescence intensities of p-AKT staining in RGCs). Values are represented as mean ± standard error of the mean (SEM), n = 3. Scale bar indicates 20 µm.