Appendix 2 of Phatak, Mol Vis 2016; 22:1048-1061.
To access the data, click or select the words “Appendix 2.” Immunoreactivity and specificity of Bcl-xL antibody. The specificity and immunoreactivity of the Bcl-xL antibody was tested by a immunoblot analysis of optic nerve extracts from Brown Norway rats. Briefly, optic nerve extracts obtained from the left (L) and right (R) eyes of a Brown Norway rat were run on a SDS–PAGE gel, and transferred to a nitrocellulose membrane. Primary antibody incubation was performed with a rabbit anti-Bcl-xL antibody and secondary antibody incubation was done with a donkey anti-rabbit IgG Horse radish peroxidase (HRP) conjugate. The blots were developed with an enhanced chemiluminescence kit. A single doublet band (30 kDa) was observed indicating the specificity of the Bcl-xL antibody used in this study.