Molecular Vision: Libert, Mol Vis 2016; 22:100-115. Table 5

Table 5. Dose–response curve of hyperosmolar stress on TonEBP nuclear translocation in ARPE-19 cells.

Condition	4 h	12 h
Iso-osmolar	0.00±0.00%	0.00±0.00%
Na25	0.67±1.15%	71.67±9.61%#
Na50	6.33±5.86%	89.67±5.51%#
Na100	77.67±17.50%*	100.00±0.00%#
Su50	2.00±2.65%	67.00±7.00%#
Su100	6.67±4.04%	83.33±9.07%#
Su200	46.00±14.73%*	100.00±0.00%#

ARPE-19 cells were incubated for 4 h or 12 h with iso-osmolar or hyperosmolar (Na25, Na50, Na100, Su50, Su100, Su200) medium. Cells were then fixed and exposed to immunofluorescent staining of TonEBP as described under Material and Methods. The percentage of ARPE-19 cells displaying a nuclear localization of TonEBP protein was determined by cell count following immunofluorescent staining. Data represent the mean ± SEM (n=3) and are expressed as the percentage of ARPE-19 cells displaying a nuclear localization of TonEBP protein. For both NaCl and sucrose stimulation, statistical analyses were performed using repeated measures ANOVA and Dunnett’s post-hoc tests. * and # indicate p<0.05 statistical significance compared to Iso-osmolar condition at 4h and 12h, respectively.