Molecular Vision: Libert, Mol Vis 2016; 22:100-115. Table 3

Table 3. Kinetics of TonEBP nuclear translocation in ARPE-19 cells exposed to hyperosmolar stress.

Condition	4 h	8 h	12 h
Iso-osmolar	0.00±0.00%	0.67±1.15%	0.33±0.58%
Na100	70.67±5.86%*	80.33±11.93%&	100.00±0.00%#
Su200	60.33±5.51%*	81.67±10.07%&	100.00±0.00%#

ARPE-19 cells were incubated for 4, 8 or 12 h with iso-osmolar or hyperosmolar (Na100 or Su200) medium. Cells were then fixed and exposed to immunofluorescent staining of TonEBP as described under Material and Methods. The percentage of ARPE-19 cells displaying a nuclear localization of TonEBP protein was determined by cell count following immunofluorescent staining. Data represent the mean ± SEM (n=3) and are expressed as the percentage of ARPE-19 cells displaying a nuclear localization of TonEBP protein. Statistical analyses were performed using repeated measures ANOVA and Dunnett’s post-hoc tests. p<0.05 compared to Iso-osmolar condition at 4h: *; 8 h: &; 12 h: #.