Molecular Vision: Libert, Mol Vis 2016; 22:100-115. Table 2

Table 2. Kinetics of TonEBP protein expression in ARPE-19 cells exposed to hyperosmolar stress.

Condition	Iso-osmolar	Na100	Su200
1h	100.0±0.0%	96.3±4.6%	101.0±2.6%
2h	100.3±5.0%	102.7±3.8%	98.3±1.3%
4h	100.3±0.3%	99.7±6.3%	114.7±4.1%
8h	100.7±0.7%	116.0±8.5%	111.3±7.2%
12h	99.7±0.9%	113.0±0.6%*	112.7±1.5%*
24h	99.3±0.7%	112.7±2.2%*	120.7±4.9%*

ARPE-19 cells were incubated for 1, 2, 4, 8, 12 or 24h with iso-osmolar or hyperosmolar (Na100, Su200) medium. TonEBP protein levels were determined by semiquantitative western blot analysis. β-actin was used as an internal control of protein expression. Band densities were estimated using an imaging system as described under Material and Methods. TonEBP protein level (ratio of TonEBP band density over β-actin band density) was expressed as percent of the control value (iso-osmolar condition, set to 100%) and are the mean ± SEM (n=3). For both NaCl and sucrose stimulation, statistical analyses were performed using repeated measures ANOVA and Dunnett’s post-hoc tests.* indicates p<0.05 compared to Iso-osmolar condition at similar incubation time.