Table 2 of Lo, Mol Vis 2016; 22:1-8.

Table 2. Interpretation of bioburden using the signal indices of polymerase chain reaction (PCR) analysis.

Gradea Culture Indices of PCRb analysis
Bioburden
(CFU/ml) cPCR-dc
(gray level) cPCR-rd
(a.u.e)
Rare ≤200 ≤8 ≤1.19
Light 201–2000 9–15 1.20–1.35
Moderate 2001–12000 16–28 1.36–1.61
Heavy ≥12,001 ≥29 ≥1.62

aGrade of bacterial contamination. bPCR, polymerase chain reaction gel electrophoresis. ccPCR-d, corrected difference in the PCR band intensity from the background. dcPCR-r, corrected ratio in the PCR band intensity from the background. ea.u., arbitrary unit.

Lo, Mol Vis 2016; 22:1-8. http://www.molvis.org/molvis/v22/1

©2016 Molecular Vision http://www.molvis.org/molvis/

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