Table 1 of
Ambreen, Mol Vis 2015; 21:985-999. Table 1 of
Ambreen, Mol Vis 2015; 21:985-999.
Table 1. Primer sequence and PCR optimization conditions with restriction enzymes.
| Gene variants | rs number | Primer sequence | uncut | cut | Restriction enzymes | Annealing temperature (˚C) | |
|---|---|---|---|---|---|---|---|
| IL-6 174 G > C | 1800795 | Forward: 5' TGACTTCAGCTTTACTCTTTGT3' | 198 | G: 198 | SfaN1 | 56.0 | |
| Reverse: 5'CTGATTGGAAACCTTATTAAG3' | C:144 | ||||||
| IL-6 572 C > G | 1800796 | Forward: 5'GCAAAGTCCTCACTGGGAGGA3' | 296 | C: 296 | BsrB1 | 64.0 | |
| Reverse: 5'TCTGACTCCATCGCAGCCC3' | G: 202 | ||||||
| IL-6 597 A > G | 1800797 | Forward: 5'GGAGTCACACACTCCACCTG3' | 419 | G: 419 | Fok1 | 64.0 | |
| Reverse: 5'AGCAGAACCACTCTTCCTTTACTT3' | A: 362 | ||||||
| IL-8 251 A > T | 4073 | Forward: 5′TCATCCATGATCTTGTTCTAA3' | 542 | T: 542 | Mfe1 | 60.0 | |
| Reverse: 5′GGAAAACGCTGTAGGTCAGA3' | A: 449 | ||||||
| IL-8 781 C > T | 2227306 | Forward: 5′CTCTAACTCTTTATATAGGAATT3' | 203 | T: 203 | EcoR1 | 56.0 | |
| Reverse: 5′GATTGATTTTATCAACAGGCA3' | C: 185 | ||||||
| IL-8 1633 C > T | 2227543 | Forward: 5′CTGATGGAAGAGAGCTCTGT3' | 397 | T: 397 | NlaIII | 60.0 | |
| Reverse: 5′TGTTAGAAATGCTCTATATTCTC3' | C: 233 | ||||||
| VEGF 936 C > T | 3025039 | Forward 5ꞌ AAGGAAGAGGAGACTCTGCGC3ꞌ | 198 | T:198 | HpyCH4 III | 58.0 | |
| Reverse 5ꞌ TATGTGGGTGGGTGTGTCTACAG3ꞌ | C:112 | ||||||
| VEGF 2578 A > C | 699947 | Forward 5ꞌ GGATGGGGCTGACTAGGTAAGC3ꞌ | 324 | C:324 | BglII | 55 .0 | |
| Reverse 5ꞌ AGCCCCCTTTTCCTCCAAC3ꞌ | A:202 | ||||||
| CRP 1846 C > T | 1205 | Forward 5ꞌ GGAGTGAGACATCTTCTTG3ꞌ | 227 | A:227 | HpyCH4 III | 50.0 | |
| Reverse 5ꞌ CTTATAGACCTGGGCAGT3ꞌ | G:131 | ||||||
| CRP 1444 G > A | 1130864 | Forward 5ꞌ AGCTCGTTAACTATGCTGGG3ꞌ | 181 | C:181 | HpyCH4 III | 59.0 | |
| Reverse 5ꞌ CTTCTCAGCTCTTGCCTTAT3ꞌ | T:156 | ||||||