Figure 1. Agarose gel electrophoresis of PCR showing the detection of TGIF1 and TGIF2 mRNA expression in human corneal fibroblasts. Appropriately sized PCR amplification products for TGIF1 and TGIF2 were detected in two independent cDNA samples (S1 and S2) of corneal fibroblasts prepared from two separate human donor corneas. The lower level of TGIF detected in sample S2 is due to a smaller amount of starting cDNA in the PCR reaction. -ve denotes negative controls that contained TGIF1 and TGIF2 primers but water instead of cDNA. L denotes a 100-base-pair DNA ladder.