Figure 3. Gamma-aminobutyric acid_Aα1 (GABA_Aα1) and GABA_Aρ1 protein expression in cultured human RPE cells detected by western blots (A) and immunofluorescence (B) (representative image; n = 5). A: Specific bands presented at the approximate location of 51 kDa (GABA_Aα1), 48 kDa (GABA_Aρ1), and 43 kDa (β-Actin) in lysates of RPE and the retina (positive control, PC). B: Immunofluorescence staining of GABA_Aα1 and GABA_Aρ1 in cultured human RPE. Nuclei were stained by 4',6-diamidino-2-phenylindole (DAPI; blue; bar = 20 μm). NC, negative control.