Figure 4. Prediction of DNA binding residues in the PAX6 HD. Prediction of PAX6 wild-type (WT) and mutant homeodomain DNA binding residues using the BindN tool shows that the mutation of residue glutamine (Gln) to arginine (Arg) at position 255 causes contact formation with DNA. The prediction shows binding residues as “+” and non-binding residues as “-”. The confidence values are set on a scale of 0 (lowest) to 9 (highest).