Appendix 1 of Dhamodaran, Mol Vis 2015; 21:828-845.

Appendix 1. Culture and characterization of limbal, conjunctival and oral mucosal biopsies.

Explants from all the three tissue origins were cultured for 14 days. Day 0 explants cultures at 10X magnification (A, D, G). Day 14 cultures at lower (10X; B, E, H) and higher (40X; C, F, I) magnification. Gel documentation image showing RT–PCR results for characterizing the cells of limbal (J), conjunctival (K) and oral (L) cultures of day 14 using primers for gapdh, cytokeratin 3, 4, 12, 13, 14, 15, connexin 43, p63α, abcg2 and vimentin. To access the data, click or select the words “Appendix 1.”