Figure 3. Quantification of Pde6b and S-opsin expression in 3-week old mice. A: Agarose gel electrophoresis of the qPCR products with Pde6b specific primers. The 359 bp Pde6b amplicon is detectable in all four mouse strains, however, at markedly different expression levels. B: Normalized Pde6b mRNA levels are significantly higher in C3H/HeOu compared to FVB/N mice, but indistinguishable in the two
Pde6b+ control strains. For each group, n=7–8 retinas from four mice. C: β-PDE immunoreactivity of retinal protein extracts from Pde6b+, rd1, and C57BL/6 wild-type mice at postnatal day 21 using an antibody specific for the N-terminal region of β-PDE. An anti-GAPDH
antibody detecting a band of approximately 37 kDa was used as a loading control. No β-PDE expression is detected in rd1 strains. D: Age-dependent S-opsin transcript levels. No significant differences exist between the two Pde6b+ lines and young, 4- to 8-week-old C3H/HeOu mice. In contrast, the S-opsin transcripts in the retinas from 4-week-old FVB/N
mice were significantly lower than in coeval C3H/HeOu retinas. For each group, n=6 retinas from three mice. Error bars indicate
standard deviation (SD). The fits are exponential with the R-square values for C3H/HeOu and FVB/N at 0.92 and 0.87, respectively.