Figure 5. Comparison of TRPP and Piezo channel expression between different tissues. Expression of TRPP1–2 and Piezo1–2 channels in the optic nerve head, brain, corpus callosum, and optic nerve proper of C57BL/6 mice was determined with conventional (A) and quantitative real-time PCR (B and C). A: TRPP1–2 and Piezo1–2 were expressed in all the tissues examined. Two splice variants of the Piezo2 gene were detected. B and C: TRPP and Piezo mRNA levels in four different tissues are presented as the fold difference relative to the same gene expression in the optic nerve head (n=6 for the ONH and ONP, n=5 for the brain and CC). Significant expression differences for each gene between four tissues were analyzed using ANOVA. *p<0.05, **p<0.01, and ***p<0.001. ONH, optic nerve head; CC, corpus callosum; ONP, optic nerve proper. Error bar, mean ± standard error of the mean (SEM).