Figure 3. Single-cell RT–PCR analysis of TRP and Piezo channel genes in single optic nerve head astrocytes. A: A representative picture of acutely dissociated astrocytes. This cell has the thick, elongated cell body and long primary processes that are the morphology of optic nerve head astrocytes observed in vivo. Scale bar, 20 μm. B: 25 transient receptor potential (TRP; seven TRPC, six TRPV, eight TRPM, one TRPA, three TRPP) and two Piezo channel genes were examined. The astrocyte in A expresses TRPC1–2, TRPV2, TRPM7, TRPP1–2, and Piezo2 channels. Glial fibrillary acidic protein (GFAP) was used as an astrocyte marker. MBP, an oligodendrocyte marker. CD45, a microglia marker. M, DNA length marker (100 bp ladder).