Figure 4. Influence of SP-1 on apical and basal VEGF-A secretion. In the RPE/choroid, the inhibition of SP-1 significantly reduces vascular endothelial growth factor (VEGF)-A at 24 h and 48 h on the apical (A) and basal sides (B). Similar results are obtained in primary RPE cell culture, with a significant decrease in VEGF-A secretion on the apical (C) and basal (D) sides, at 24 h and 48 h. Supernatants were collected for 24 h. Significance was determined with the Student t test; ++ p<0.01 +++=p<0.001. n=6. mithra=mithramycin. Bars depict the mean and the standard deviation.