**Figure 3. **Influence of NF-κB on apical and basal VEGF-A secretion. In the RPE/choroid, the inhibition of nuclear factor-kappa B (NF-κB)
significantly reduces vascular endothelial growth factor (VEGF)-A at 24 h and 48 h both on the apical (**A**) and basal sides (**B**). Similar results are obtained in primary RPE cell culture, with a significant reduction of VEGF-A secretion on the apical
(**C**) and basal (**D**) sides, at 24 h and 48 h). Supernatants were collected for 24 h and were analyzed in enzyme-linked immunosorbent assay (ELISA).
Significance was determined with the Student *t* test; + p<0.05, ++ p<0.01, +++ p<0.001. n=4 (**A**, **B**), n=6 (**C**, **D**). NF-κB=NF-κB inhibitor. Bars depict the mean and the standard deviation.