Figure 5. The mRNA expression of angiogenesis-related genes detected by real-time polymerase chain reaction (RT-PCR). All the data represented the relative fold change of mRNA expression of the genes of interest. There were statistically significant differences in the expression of VEGF, TNF-a, MMP-8, and MMP-9 between the 100 μM fasudil treated group and the phosphate-buffered saline (PBS) treated group (n=8/group, * indicates p<0.05, ** indicates p<0.01).