Figure 4. Anti-inflammatory effects of fasudil on the alkali-burned corneas. A–C: Hematoxylin-eosin staining of the murine corneas (magnification ×400). The number of infiltrated polymorphonuclear neutrophils (PMNs), macrophages, and lymphocytes in the corneal stroma per field was lower with 100 μM fasudil treatment than with phosphate-buffered saline (PBS) treatment. D–F: Immunohistochemical staining of PMNs. The endothelium of corneal vessels is indicated with red arrows. The number of infiltrated PMNs (brown staining, black arrows) in the corneal stroma per field was lower after100 μM fasudil treatment than with PBS treatment (n=7/group, ** indicates p<0.01).