Figure 6. Angiogenesis-related proteins secreted by C-FVMs: The effect of high glucose and hyperosmolarity. Map of the human angiogenesis antibody array targeting 55 proteins related to angiogenesis and angiogenesis-related factors identified 11 proteins secreted by C-FVMs from clinical case 3 (A). For identity and coordinates of all 55 proteins assayed, see Appendix 4. Protein levels of (B) angiopoietin-1, (C) thrombospondin-1, (D) PEDF, and (E) endothelin-1 under different glucose treatments were measured in human retinal pericytes (HRP) and C-FVMs from clinical case 3 using an ELISA assay. Student t tests show there was a significant reduction in levels of angiopoietin-1 and thrombospondin-1 by cells grown in high glucose (30 mM D-glucose) and under hyperosmolar conditions (30 mM L-glucose), as compared to normal glucose and normal osmolar conditions (5 mM). No changes in PEDF or endothelin-1 protein levels were observed in C-FVMs in response to changes in glucose or osmolarity conditions. The levels of angiopoietin-1, thrombospondin-1, PEDF, and endothelin-1 did not in change in HRP in response to treatment with different glucose or osmolar conditions. Error bars represent ± standard deviations; **p<0.01; n=3.