Figure 5. Isolation of CD31-positive cells from C-FVMs. Immunofluorescence localization of isolectin (A) and CD31 (B) cells from clinical case 4 identifies rare positive cells. Fibrovascular membrane (FVM) from clinical case 5 processed for immunohistochemistry using antibodies against CD31 (red) and counterstained with hematoxylin (blue; C). Light micrographs of C-FVMs from clinical case 5 show isolation of CD31-positive cells, bound to CD31-antibody coated Dynabeads before cell culture (D). Scale bar=50 µm.