Figure 5. Reformation and distribution of TJ protein ZO-1 after Y-27632 was removed. Recovery of ZO-1 expression after pretreatment of cells with Y-27632 for 1 day resulted in the gradual development of ZO-1 in the contiguous cells (A, B). The continuous pattern of the ZO-1 protein reformed abruptly at cell junctions and aligned in a hexagonal shape, after the cells were pretreated with Y-27632 for 2 days (C, D) and 3 days (E, F).