Figure 6. IGFBP-3 regulated VEGF level through PKCzeta activation. REC were treated with IGFBP-3 plasmid and PRKCzeta in REC in NG and HG media. A: Western blot data of the ratio of phosphorylated to total PKCzeta; B: Western blot results of VEGF levels; *p<0.05 versus NG control plasmid DNA transfection. ^#p<0.05 versus HG control plasmid DNA transfection. *p<0.05 versus HG IGFBP-3 plasmid DNA transfection. ^$p<0.05 versus HG IGFBP-3 control DNA transfection + PRKCzeta. n=3. Data are mean ± SEM.