Figure 3. IGFBP-3 regulates PKCzeta activation and VEGF levels through eNOS. REC were treated with IGFBP-3 plasmid and recombinant eNOS in REC in both NG and HG media. A: Western blot data of phosphorylated and total PKCzeta levels; B: Western blot results of VEGF levels; *p<0.05 versus NG control plasmid DNA transfection. ^#p<0.05 versus HG control plasmid DNA transfection. *p<0.05 versus HG IGFBP-3 plasmid DNA transfection. ^$p<0.05 versus HG control DNA transfection + eNOS. n=3. Data are mean ± SEM.