Figure 2. Quantification of TGF-βR1, TGF-βR2, and TGF-βR3 expression in HCFs and HKCs following stimulation with all three TGF-β isoforms. RT-PCR analysis shows gene expression for (A) TGF-βR1, (B) TGF-βR2, and (C) TGF-βR3. Western blot analysis shows protein expression for (D) TGF-βR1, (E) TGF-βR2, and (F) TGF-βR3. G: Representative Western blots from three independent experiments. All samples were repeated at least three times. p<0.05 was considered to be statistically significant (****p<0.0001, ***p<0.001, *p<0.05).