Figure 1. Quantification of TGF-β1, TGF-β2, and TGF-β3 expression in HCFs and HKCs following stimulation with all three TGF-β isoforms. RT-PCR analysis shows gene expression for (A) TGF-β1, (B) TGF-β2, and (C) TGF-β3. Western blot analysis shows protein expression for (D) TGF-β1, (E) TGF-β2, and (F) TGF-β3. G: Representative Western blots from three independent experiments. All samples were repeated at least three times. p<0.05 was considered to be statistically significant (*p<0.05).